Table 2.
Amplicon sizes and restriction endonuclease patterns of gN genomic variants
| gN genotype and PCR product size (bp) | (*) Fragment sizes (bp) | ||
|---|---|---|---|
| SacI | ScaI | SalI | |
| 1 (420) | 297, 123 | 420 | 420 |
| 2 (417) | 229, 123, 65 | 417 | 296, 121 |
| 3a (420) | 420 | 420 | 420 |
| 3b (420) | 420 | 221, 172, 27 | 420 |
| 4a (414) | 291, 123 | 221, 166, 27 | 341, 73 |
| 4b (414) | 414 | 387, 27 | 341, 73 |
| 4c (414) | 411 | 239, 172 | 338, 73 |
Pignatelli et al. [22]
(*) For each of the glycoprotein gene amplification products, the restriction pattern was obtained upon digestion with the endonuclease combinations indicated in the related panels