Table 3. Queries identified as unique peptides of each myosin heavy chain (MHC) isoform.
| MHC | Sequence | Residue number | Ion scorea) | MW (expt) | MW (calc) |
|---|---|---|---|---|---|
| MHC-1 | TSVFVADPKESFVKATVQSR | 36-55 | 62 | 2197.6 | 2196.5 |
| MHC-1 | VGNEFVTKGQTVEQVYNAVGALAK | 409-432 | 54 | 2523.2 | 2522.8 |
| MHC-1 | GQTVEQVYNAVGALAK | 417-432 | 71 | 1648.6 | 1647.8 |
| MHC-1 | SSVKTLALLFSGPASGEAEGGPK | 615-637 | 60 | 2203.4 | 2203.4 |
| MHC-1 | ARLQTESGEFSR | 1281-1292 | 71 | 1380.1 | 1380.5 |
| MHC-1 | LQTESGEFSRQLDEKDALVSQLSR | 1283-1306 | 83 | 2737.8 | 2736.9 |
| MHC-1 | QLDEKDALVSQLSR | 1293-1306 | 88 | 1602.9 | 1601.8 |
| MHC-1 | LAQRLQDAEEHVEAVNAK | 1396-1413 | 44 | 2021.9 | 2021.2 |
| MHC-1 | LQDAEEHVEAVNAKCASLEK | 1400-1419 | 51 | 2242.1 | 2241.4 |
| MHC-1 | QKYEETHAELEASQKESR | 1461-1478 | 44 | 2163.9 | 2163.3 |
| MHC-1 | IVESMQSTLDAEIR | 1596-1609 | 49 | 1607.8 | 1607.8 |
| MHC-1 | SYKRQAEEAEEQSNVNLSK | 1880-1898 | 82 | 2210.2 | 2210.3 |
| MHC-1 | RQAEEAEEQSNVNLSK | 1883-1898 | 64 | 1832.4 | 1831.9 |
| MHC-1 | QAEEAEEQSNVNLSK | 1884-1898 | 90 | 1676.6 | 1675.7 |
| MHC-2 | SAETEKEMATMKEEFQK | 847-863 | 47 | 2050.0 | 2049.2 |
| MHC-2 | LQTESGEFSRQLDEKEALVSQLSR | 1285-1308 | 60 | 2751.5 | 2751.0 |
| MHC-2 | QLDEKEALVSQLSR | 1295-1308 | 52 | 1615.5 | 1615.8 |
| MHC-2 | ALSKANTEVAQWR | 1365-1377 | 58 | 1473.5 | 1473.6 |
| MHC-2 | ANTEVAQWR | 1369-1377 | 69 | 1074.3 | 1074.1 |
| MHC-2 | LQAAEEHVEAVNAK | 1402-1415 | 80 | 1508.4 | 1508.6 |
| MHC-2 | RQAEEAEEQSNTNLSK | 1885-1900 | 59 | 1834.5 | 1833.9 |
| MHC-2 | QAEEAEEQSNTNLSK | 1886-1900 | 73 | 1677.4 | 1677.7 |
| MHC-7 | DVFVPDDKEEFVKATILSR | 36-54 | 46 | 2209.1 | 2208.5 |
| MHC-7 | EQATGKGTLEDQIIQANPALEAFGNAK | 208-234 | 81 | 2815.4 | 2815.1 |
| MHC-7 | GTLEDQIIQANPALEAFGNAK | 214-234 | 80 | 2199.5 | 2200.4 |
| MHC-7 | VGNEYVTKGQNVQQVVYAK | 406-424 | 69 | 2124.6 | 2124.4 |
| MHC-7 | GQNVQQVVYAK | 414-424 | 58 | 1234.2 | 1233.4 |
| MHC-7 | LFDNHLGK | 552-559 | 51 | 943.8 | 943.1 |
| MHC-7 | ILNPAAIPEGQFIDSR | 724-739 | 69 | 1740.4 | 1741.0 |
| MHC-7 | LKEALEKSEAR | 859-869 | 60 | 1273.4 | 1273.4 |
| MHC-7 | ALQEAHQQALDDLQAEEDKVNTLTK | 998-1022 | 48 | 2809.0 | 2809.0 |
| MHC-7 | KKHADSVAELSEQIDNLQR | 1194-1212 | 67 | 2182.0 | 2181.4 |
| MHC-7 | SKAEETQRSVNDLTSQR | 1261-1277 | 47 | 1950.2 | 1949.0 |
| MHC-7 | SVNDLTSQRAK | 1269-1279 | 45 | 1218.2 | 1218.3 |
| MHC-7 | LQTENGELSR | 1280-1289 | 55 | 1146.1 | 1146.2 |
| MHC-7 | QLDEKEALISQLTR | 1290-1303 | 48 | 1643.8 | 1643.8 |
| MHC-7 | VLSKANSEVAQWR | 1360-1372 | 69 | 1487.5 | 1487.7 |
| MHC-7 | LAQRLQDAEEAVEAVNAK | 1393-1410 | 54 | 1955.6 | 1955.1 |
| MHC-7 | SNAAAAALDKK | 1435-1445 | 44 | 1059.3 | 1059.2 |
| MHC-7 | VVDSLQTSLDAETR | 1593-1606 | 57 | 1533.3 | 1533.6 |
| MHC-7 | VRELENELEAEQKR | 1819-1832 | 51 | 1743.4 | 1742.9 |
| MHC-7 | QAEEAEEQANTNLSK | 1881-1895 | 98 | 1662.3 | 1661.7 |
| MHC-7 | VQHELDEAEERADIAESQVNKLR | 1899-1921 | 77 | 2680.2 | 2679.9 |
a)Individual ions scores greater than 43 indicate identity (p<0.05).