5F 203 activates JNK and p38 signaling in sensitive breast cancer cells. (A) MDA-MB-468 and T47D cells were exposed to 1 µM 5F 203 at varying time points before being harvested and analyzed by immunoblotting for JNK, p38, or ERK phosphorylation. Data is representative of three independent experiments. Gel is of a representative blot, and sections within the blot were rearranged based on treatments specified. (B) Graphs based on densitometry determination ratios for phospho-proteins relative to total proteins relative to time 0 h measurements (fold change). *p < 0.05, **p < 0.01, and ***p < 0.001 in comparison to time 0 h measurements.