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. Author manuscript; available in PMC: 2015 Nov 27.
Published in final edited form as: Colloids Surf B Biointerfaces. 2013 Aug 1;112:108–112. doi: 10.1016/j.colsurfb.2013.07.045

Figure 3. PEI coating enhances cellular uptake of BT.

Figure 3

(A) BT or BT-PEI at a weight ratio of polymer to NP of 1:40 were added to HeLa cells and incubated for 3 hours prior to fixing and propidium iodide (PI) staining. PI signal allows for discernment of cell boundaries and SHG indicates the signal from the BT particles. Colocalization between these two channels is shown in the rightmost panel to identify internalized particles. Cell outlines are shown in yellow. (B) BT or BT-PEI particles were counted in 4 different fields of view each to determine an average number of internalized NPs per cell. * p < 0.01 (student’s t test). Scale bars represent 10 µm.