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. Author manuscript; available in PMC: 2015 Nov 27.
Published in final edited form as: J Biol Methods. 2015 Nov 17;2(4):e31. doi: 10.14440/jbm.2015.96

Figure 1.

Figure 1

A. Scheme of 24-well preparation and cell plating into 3D lrECM. Wells are first coated with agarose to prevent cell attachment. After trypsinization and counting, cell suspension is diluted with lrECM and placed into the wells. Medium is added after lrECM mixture has polymerized. B. Prior to cell lysis, redundant medium/lrECM is carefully removed without disturbing the cell layer. Cell lysates are homogenized with a 27 gauge needle and centrifuged. The supernatant is stored at −80°C.