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. 2015 Jul 31;6(22):18790–18799. doi: 10.18632/oncotarget.4618

Figure 2. Bmk-1 over-expression extends lifespan and bmk-1 inhibition by RNAi shortens lifespan in C. elegans.

Figure 2

A. qRT-PCR validation of bmk-1 over-expression worm lines shows increased bmk-1 gene expression (* indicates p < 0.01). Y-axis represents the relative expression level of bmk-1 normalized to act-1, and n ≥ 10 for each group. B. lifespan measurement for both WT (GFP-expressing, n = 126) and bmk-1 over-expressing (n = 161) animals. Both median lifespan and maximum lifespan of bmk-1 over-expressing lines show a 25% extension when compared to WT lines (p < 0.001, p values were derived from student t-test and log-rank test). C. qRT-PCR validated the RNAi effect, indicating a significant reduction (∼64%) of bmk-1 transcripts in WT worms treated with bmk-1 RNAi. Y-axis represents the relative expression level of bmk-1 normalized to act-1, and n ≥ 10 for each group. * indicates p < 0.01. D. A shortened lifespan, both median (32%) and maximum (15%), was induced by bmk-1-specific RNAi in C. elegans (n = 82), as compared to RNAi vector lines (n = 75) (p < 0.0001).