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. 2015 Jun 1;6(22):19006–19016. doi: 10.18632/oncotarget.4147

Figure 4. Knockdown of ETV1 expression enhances the migration of melanoma cells.

Figure 4

A. Transfection of siRNA against ETV1 was performed in the indicated melanoma cell lines. mRNA levels of ETV1 was confirmed with qPCR. Figure shows the mean of 3 independent experiments performed; B. ETV1 expression at the protein level was determined by Western blot following transfection with scrambled sequence (mock) or siRNA against ETV1 in the indicated melanoma cell lines. Actin levels served as control. Normalized densitometry values of ETV1 relative to actin are indicated. A representative blot is shown out of 3 experiments performed; C-D. Migration of transfectants was quantified by measurement of electric impedance differences of the transwell with the xCELLigence RTCA instrument. Figures represent a representative experiment out of three for each melanoma cell line. ***denotes P < 0.001 (2-tailed t-test).