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. 2015 May 22;6(22):19087–19101. doi: 10.18632/oncotarget.4236

Figure 5. HMGA1 and CCNE2 regulate YAP localization in a MST1/2 and LATS1/2 kinase-dependent manner in breast cancer cells.

Figure 5

a. Immunofluorescence images of YAP in MDA-MB-231 cells, indicating the effect of LATS1/2 and MST1/2 silencing on cells in which HMGA1 and CCNE2 were depleted. In the graph, the ratio of nuclear YAP relative to siCTRL±SD is presented (n = 3). Images were taken at X 40 magnification. b. The same experiment presented in a. performed in MDA-MB-157 cells. c. Wound-healing assay using MDA-MB-231 cells stably infected with pBABE-FLAG (FLAG) or pBABE-FLAG-YAP-5SA (FLAG-5SA) after transfection with siCTRL, siCCNE2b or siHMGA1 for 72 h. The data are presented as the means of the percentage of wound closure relative to each control (siCTRL)±SD (n = 3). See Supplementary Figure S5c for Western blot analysis. d. Wound-healing assay using MDA-MB-231 cells treated as in panel a. *P < 0.05, **P < 0.01, ***P < 0.001, NS = not significant; two-tailed Student's t-test.