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. 2015 Jul 30;6(22):19228–19245. doi: 10.18632/oncotarget.4653

Figure 2. DCA-mediated activation of the AMPK pathway is required for p53 induction.

Figure 2

A. AML cells were incubated with the indicated concentrations of DCA or 5 mM metformin for 24 hr. DCA increases the phosphorylation levels of ACC (at Ser79), AMPKα (at Thr172) and p53 (at Ser46). B. Graph representing the ratio between phosphorylated ACC and total ACC protein levels. Bands were quantified using the Image lab software from two independent experiments. C. Time-course analysis by western blotting of the effect of 20 mM DCA in MOLM13 cells, including p53 phosphorylation on Ser15 and Ser46. D. AMPKα1 silencing with two different siRNAs attenuates DCA-induced p53 transcriptional activity in MOLM13 cells. Quantitative RT-PCR analysis of p21 and GLS2 mRNA levels (values are the mean ± SEM of two independent experiments performed in triplicate). E. AMPKα1 silencing decreases DCA-induced phosphorylation of ACC (Ser79) and p53 (Ser46). See also Supplementary Figure S2.