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. 2015 May 20;6(22):19279–19289. doi: 10.18632/oncotarget.4220

Figure 4. Tracking TP53 mutated tumor cells in p53 expressing cells by laser-microdissection.

Figure 4

A. In the RCC lung metastasis sample, p53-expressing cells are laser-microdissected for further molecular analyses. B. TP53 status is assessed by PCR-HRM screening on exons 5 to 8 in p53-expressing cells microdissected from the primary RCC (PRCC), the two xenografts derived from it (Xe1, Xe2), and the lung metastasis (CM). A shift on exon 6 is identified in the four samples when compared to wild-type profile (WT). C. Sequencing of exon 6 of TP53 identifies an identical missense mutation c.605G>A p.R202H in laser-microdissected p53-expressing cells from the primary RCC, in the two tumor xenografts derived from it, and in the lung metastasis.