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. Author manuscript; available in PMC: 2015 Nov 27.
Published in final edited form as: Cancer Res. 2014 Feb 13;74(7):2073–2081. doi: 10.1158/0008-5472.CAN-13-2993

Figure 1. Development of an iHPV-Luc transgene.

Figure 1

(A) Scheme for iHPV-Luc transgene. A floxed EGFP reporter was placed downstream from the CAG promoter to inhibit expression of the E6E7-IRES-Luc bicistronic cassette. Excision of the floxed EGFP cassette resulted in induction of the E6E7-IRES-Luc gene cassette. (B–D) Cre recombination of iE6E7 caused decreased EGFP (grey histograms) (B), and increased E6 expression (C) and luciferase activity (D). The iHPV-Luc construct was transfected into HEK cells with or without a Cre recombinase expression vector. Transfected cells were assessed for EGFP fluorescence using flow cytometry, E6 expression by Western analysis and luciferase activity. Data represented two separate experiments performed in duplicate.