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. Author manuscript; available in PMC: 2015 Nov 27.
Published in final edited form as: Cancer Res. 2014 Feb 13;74(7):2073–2081. doi: 10.1158/0008-5472.CAN-13-2993

Figure 3. Generation of KHR mice that recombine the iHPV-Luc and LSL-Kras genes upon Tam treatment.

Figure 3

KH mice and LSL-Kras transgenic mice were bred to generate offspring containing all combinations of the transgenes. (A) PCR confirmed the genotype of KHR mice containing the K14-CreERtam × LSL-Kras × iHPV-Luc transgene. Transgenic mice: KH: K14-CreERtam × iHPV-Luc; R: LSL-Kras; KR: K14-CreERtam × LSL-Kras; KHR: K14-CreERtam × iHPV-Luc × LSL-Kras. (B) Treatment of these offspring with Tam resulted in excision of the iHPV-Luc and LSL-Kras transgenes. (C) After tamoxifen treatment, oral tumors grew faster in KHR mice than in KR mice. Similar results were observed in two independent experiments. Symbols indicate individual mice. Significant differences between KHR and KR mice were denoted by * for P < .05 and ** for P < .001. (D) Oral tumors arising in KHR mice were positive for p16 and MCM7, two biomarkers for HPV-positivity.