Table 3.
Chemical specific parameters for adult rat PFOA PBPK model.
| Parameter | Definition | Units | Value | Source |
|---|---|---|---|---|
| Free | free fraction of PFOA in plasma | unitless | 0.09 | Fit to Kemper, Experiment 6b and 6b |
| Vmax_basoC | Vmax of basolateral transporters measured in in vitro studies (average of Oat1 and Oat3) | mg/h/kg BW0.75 | 0.04 | Calculated from Nakagawa et al., 2007 |
| Km_baso | Km of basolateral transporters (Oat1 and Oat3) | ug/mL | 27.20 | Nakagawa et al., 2007 |
| Vmax_apicalC | Vmax of apical transporters measured in in vitro studies (Oatp1a1) | mg/h/kg BW0.75 | 0.947 | Calculated from Nakagawa et al., 2007 |
| Km_apical | Km of apical transporters (Oatp1a1) | ug/mL | 52.3 | Weaver et al., 2010 |
| RAFapi | relative activity factor of apical transporters (male) | unitless | 35.0 | Fit to Kemper, Experiment 6b |
| relative activity factor of apical transporters (female) | unitless | 0.001356 | Fit to Kemper, Experiment 6a | |
| RAFbaso | relative activity factor of basolateral transporters (male) | unitless | 4.07 | Fit to Kemper, Experiment 6b |
| relative activity factor of basolateral transporters (female) | unitless | 0.01356 | Fit to Kemper, Experiment 6a | |
| PL | liver:blood partition coefficient | 2.2 | Kudo et al., 2007 | |
| PK | kidney:blood partition coefficient | 1.05 | Kudo et al., 2007 | |
| PR | Rest of body:blood partition coefficient | 0.11 | Kudo et al., 2007 | |
| k0c | rate of absorption of PFOA in stomach | /hr/kg0.25 | 1.0 | Fit to Kemper, Experiment 1 and Experiment 6a |
| kabsc | rate of absorption of PFOA in small intestines | /hr/kg0.25 | 2.12 | Fit to Kemper, Experiment 1 and Experiment 6a |
| kunabsc | rate of unabsorbed dose to appear in feces | /hr/kg0.25 | 7.06 × 10−5 | Fit to Kemper, Experiment 1 and Experiment 6a |
| keffluxc | rate of clearance of PFOA from proximal tubule cells into blood | /hr/kg0.25 | 2.49 | Fit to Kemper, Experiment 6a and 6b |
| kbilec | biliary elimination rate | /hr/kg0.25 | 0.004 | Fit to Kemper, Experiment 1 and Experiment 6a |
| kurinec | urinary elimination rate | /hr/kg0.25 | 1.6 | Fit to Kemper, Experiment 6a and 6b |