Figure 6. IL-1 signaling is required in stromal cells for LCWE-induced coronary vasculitis.
CD45.1 WT (Ly5.1) or Il1r1−/− were irradiated (7.5Gy) followed by bone marrow transplantation to create chimeric mice (WT→WT, Il1r1−/−→WT, and WT→Il1r1−/−). 8 weeks after irradiation and transplantation the mice were i.p injected with LCWE. Two weeks later the mice were sacrificed and the hearts were analyzed by H&E. (A) Representative histology, (B) Heart vessel inflammation score, (C) incidence of KD lesions, and (D) and myocardial inflammation score were evaluated as described in Methods. Data shown are mean±SE and were compared by the normalized One way ANOVA with Tukey’s post hoc test (B and D) and Fisher exact test for incidence (C). A probability value of P≤0.05 was considered statistically significant. The scale bar is 250μm.