Skip to main content
. Author manuscript; available in PMC: 2017 May 2.
Published in final edited form as: Virus Res. 2015 May 27;216:55–65. doi: 10.1016/j.virusres.2015.05.012

Fig. 2. Schematic diagram of RRP (A) and scMP-12 systems (B).

Fig. 2

(A) RRP is produced by transfecting plasmid encoding 78kDa/NSm/Gn/Gc proteins into BHK-rep cells stably supporting the replication of L RNA and S-GFP RNA. Inoculation of RRP into naïve cells results in transcription/replication of L and S-GFP RNA and expression of L, N, and GFP proteins. (B) scMP-12 is generated in BSR-T7/5 cells stably expressing T7 polymerase by co-transfection of plasmids, each encoding L, N, and Gn/Gc proteins, L RNA, S RNA and M RNA encoding Gc mutant. Released scMP-12 contains viral RNAs and is competent for initiation of infection, as it carries wt Gn/Gc derived from the protein expression plasmid. scMP-12 is further amplified in Vero-G cells stably expressing wt Gn/Gc. Inoculation of scMP-12 into naïve cells results in viral RNA synthesis, expression of viral proteins, and production of non-infectious VLPs.