Table 2.
Gn mutants: fusion competence and efficiency of VLP production
| Amino acid sequence (820 to 837) | Fusion competence | Gc mAb recognition |
VLP production | |
|---|---|---|---|---|
| wtGc | GWGCGCFNVNPSCLFVHT | Competent | Positive | ++ |
| G820N | NWGCGCFNVNPSCLFVHT | Competent | NT | NT |
| G820S | SWGCGCFNVNPSCLFVHT | Competent | NT | NT |
| W821N | GNGCGCFNVNPSCLFVHT | Defective | Negative | NT |
| W821R | GRGCGCFNVNPSCLFVHT | Defective | Negative | NT |
| W821S | GSGCGCFNVNPSCLFVHT | Competent | Negative | NT |
| W821D | GDGCGCFNVNPSCLFVHT | Defective | Negative | NT |
| G822S | GWSCGCFNVNPSCLFVHT | Competent | NT | NT |
| G822D | GWDCGCFNVNPSCLFVHT | Defective | Positive | + |
| G822N | GWNCGCFNVNPSCLFVHT | Defective | NT | − |
| C823A | GWGAGCFNVNPSCLFVHT | Defective | Positive | − |
| C823N | GWGNGCFNVNPSCLFVHT | Defective | Positive | − |
| G824D | GWGCDCFNVNPSCLFVHT | Competent | NT | NT |
| C825A | GWGCGAFNVNPSCLFVHT | Defective | Negative | NT |
| F826N | GWGCGCNNVNPSCLFVHT | Defective | Positive | − |
| F826L | GWGCGCLNVNPSCLFVHT | Competent | NT | NT |
| F826S | GWGCGCSNVNPSCLFVHT | Defective | Positive | ++ |
| N827A | GWGCGCFAVNPSCLFVHT | Defective | Positive | ++ |
| V828N | GWGCGCFNNNPSCLFVHT | Competent | Positive | NT |
| P830N | GWGCGCFNVNNSCLFVHT | Competent | Positive | NT |
| H778A | Defective | Positive | ++ | |
| L779A | Defective | Positive | ++ | |
| H857A | NT | NT | − | |
| H857R | Defective | Positive | − | |
| H1087A | NT | NT | + | |
| H1087R | Competent | Positive | NT |
NT : Not tested
− : Under detactable level