Figure 2.

Multicolor fate mapping in olfactory neurogenesis. (A) In initial experiments, c-KitCre^ERT2/+; R26R-confetti mice were given 0.5 mg tamoxifen at p2 and euthanized at 2 weeks. All four XFPs were identified, with high density labeling precluding clonal assignment of labeled cells. (B, C) Adult mice were lesioned with methimazole and given 1 mg tamoxifen during epithelial reconstitution followed by euthanasia at 14 days. Individual single color non-overlapping XFP-labeled cell clusters were identifiable. At high magnification (B), fine cellular detail allowed definitive assessment of cell type. Here, 5 neurons are labeled by RFP. Low magnification overview demonstrates sparse labeling, permitting clonal assessment. Here, there is a single RFP+ and single nuclear-localized GFP+ clone well separated along a turbinate epithelium (arrows). (D) Quantification indicates that mean clone size and distribution is not different among different XFP-labeled clones. ANOVA for average clone size and Kruskall Wallis test for clone size distributions confirm no statistical differences, n=3 mice per time point. Bar = 25 μm in A, 10 μm in B, 50 μm in C; dashed line indicates basal lamina in A and B.