Figure 2. RNA and protein degradation rates conditioned on cell size.

A Single-cell SSA1^P-Y-GFP expression measured by flow cytometry in one replicate (37 ° C) plotted against forward scatter. The same forward scatter bin parameters were used across all experiments. B Protein decay constants were obtained by tracking bin-specific (color-coded to match A, above) decay in the presence of the translation-blocking agent cycloheximide. RNA decay constants were obtained by tracking transcriptionally-mediated inhibition of SSA1 expression after heat shock, and constrained by the protein decay rate. C Estimated protein (left column) and RNA (right column) decay constants for each forward scatter bin. Red lines are median rate constant estimates, blue lines are 25^th and 75^th percentiles, and whiskers mark the full range. Gray regions indicate decay constants estimated on the bulk data rather than binned by forward scatter (99% CI). For SSA1^P-GFP protein degradation (bottom left), the green region represents the 99% CI for expected degradation assuming dilution from growth alone. See also Figure S2.