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. Author manuscript; available in PMC: 2015 Nov 27.
Published in final edited form as: Biochemistry. 2008 May 6;47(21):5814–5822. doi: 10.1021/bi7014234

Figure 1.

Figure 1

Proteinase K protection assay of Cry1Ab wt and its mutants. Reaction was run on 4–20% SDS–PAGE gels, and the membranes were blotted using anti-1A polyclonal antibody and HRP tagged anti rabbit secondary antibody. Lane1: Pure Cry1Abwt (10 μg). Lane 2: proteinase K treated BBMV bound to Cry1Ab wt. Lane 3: Pure 1AbV171C (10 μg). Lane 4: proteinase K treated BBMV bound to 1AbV171C. Lane 5: Pure 1AbF371C (10 μg). Lane 6: proteinase K treated BBMV bound to 1AbF371C. Lane 7: Pure 1AbF371A/V171C (10 μg). Lane 8: proteinase K treated BBMV bound to 1AbF371A/V171C.