. Author manuscript; available in PMC: 2015 Nov 27.

Published in final edited form as: Biochemistry. 2008 May 6;47(21):5814–5822. doi: 10.1021/bi7014234

Table 2.

Binding Measurements of the Toxin 1Abwt and Its Mutants to Cadherin Repeats 11 and 12 Using Surface Plasmon Resonance Analysis (Biacore)^a

sample		ka (1/Ms)	kd (1/s)	K_D (M)	χ²
1Abwt:	exp 1	6.51E+04	1.17E-03	1.79E-08	1.08
1Abwt:	exp 2	6.42E+04	1.21E-03	1.89E-08	0.859
1AbV171C:	exp 1	7.64E+04	1.84E-03	2.41E-08	6.02
1AbV171C:	exp 2	7.04E+04	1.55E-03	2.21E-08	1.1
1AbF371C:	exp 1	3.66E+04	1.79E-03	4.89E-08	5.38
1AbF371C:	exp 2	3.83E+04	1.83E-03	4.78E-08	6.32
1AbF371A/V171C:	exp 1	1.83E+04	1.25E-03	6.80E-08	6.58
1AbF371A/V171C:	exp 2	1.83E+04	1.17E-03	6.41E-08	2.15

The results are expressed as K_D values (ratio of the kd:ka) and are expressed in molar units. Sets of measurements for each sample were performed twice, as shown.