Skip to main content
. Author manuscript; available in PMC: 2015 Nov 27.
Published in final edited form as: FEMS Microbiol Lett. 2009 Apr 27;295(2):156–163. doi: 10.1111/j.1574-6968.2009.01583.x

Fig. 2.

Fig. 2

Coomassie blue-stained SDS-10% PAGE of protoxins (a) and toxins after digestion with trypsin (b) and chymotrypsin (c) of Cry19Aa and muteins expressed in Bacillus thuringiensis 4Q7 cells. Lane 19, Cry19Aa; lane 1, Y410A; lane 2, E411A; lane 3, Y412A; lane 4, I413A; lane 5, Y414A; lane 6, W416A; lane 7, D418A; lane 8, V420A; lane 9, T484A; lane 10, F485A; lane 11, Y356A; lane 12, W357A; lane 13, Y324A; lane 14, F325A; lane 15, T390A; lane 16, T610A; lane M, Precision plus protein™ standards (Bio-Rad). Molecular mass marker positions (in kDa) are indicated to the left.