Table 2.
Cycling steps | E1 | E2 | E3 | E4 | E5 |
---|---|---|---|---|---|
Initial denaturation | 94 °C/5 min | 94 °C/5 min | 94 °C/5 min | 94 °C/5 min | 94 °C/5 min |
Denaturation | 94 °C/1 min | 94 °C/1 min | 94 °C/1 min | 94 °C/1 min | 94 °C/1 min |
Annealing | 61 °C/50 s | 62 °C/50 s | 63 °C/50 s | 62 °C/40 s | 58 °C/1 min |
Extension | 72 °C/50 s | 72 °C/50 s | 72 °C/50 s | 72 °C/50 s | 72 °C/50 s |
Final extension | 72 °C/5 min | 72 °C/5 min | 72 °C/5 min | 72 °C/5 min | 72 °C/5 min |
Number of cycles | 35 | 35 | 35 | 35 | 35 |
PCR: Polymerase chain reactions, SOD1: Superoxide dismutase 1