Table 2.

Thermocycler conditions for polymerase chain reactions (PCR) of five exons (E1-E5) of superoxide dismutase 1 (SOD1)

Cycling steps	E1	E2	E3	E4	E5
Initial denaturation	94 °C/5 min	94 °C/5 min	94 °C/5 min	94 °C/5 min	94 °C/5 min
Denaturation	94 °C/1 min	94 °C/1 min	94 °C/1 min	94 °C/1 min	94 °C/1 min
Annealing	61 °C/50 s	62 °C/50 s	63 °C/50 s	62 °C/40 s	58 °C/1 min
Extension	72 °C/50 s	72 °C/50 s	72 °C/50 s	72 °C/50 s	72 °C/50 s
Final extension	72 °C/5 min	72 °C/5 min	72 °C/5 min	72 °C/5 min	72 °C/5 min
Number of cycles	35	35	35	35	35

PCR: Polymerase chain reactions, SOD1: Superoxide dismutase 1