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. 2015 Jul-Sep;10(3):366–380.

Table 2.

Proteins identified using LC/MS analysis

Expression pattern. f
Cutaneous isolate (CI)
Viscerotropic isolate (VTI)
Spot
No. a 		Protein name Accession
No. b 		(PI/MW)
Exp. c 		(PI/MW)
Theo. d 		Protein Score. e Ave %vol Sd Ave %vol Sd Induction factor (V/C). g
28 hypothetical protein Q4QFW1 6.38/16 6.1/15 162 0.224 0.019 0.114 0.032 0.51
36 hypothetical protein Q4QFW1 6.3/16 6.1/15 79 0.037 0.007 0.000 0.000 NDS
44 ADF/Cofilin E9ADQ2 5.77/15 5.6/16 219 0.526 0.097 0.109 0.189 0.21
50 60S ribosomal protein L30 E9AEK1 5.29/15 9.7/11 43 0.000 0.000 0.027 0.009 DS
58 hypothetical protein, conserved (Alba superfamily) * Q4QGA9 5.12/16 5.2/13 409 0.238 0.014 0.089 0.043 0.37
64 60S ribosomal protein L23a, putative Q4QJ20 6.64/19 10.5/16 172 0.039 0.010 0.117 0.017 3.02
70 hypothetical protein, conserved (Sfi1 spindle body protein) * Q4Q6E8 4.86/20 10.6/121 72 0.000 0.000 0.107 0.021 DS
81 elongation factor 1-alpha Q4QEI9 4.96/18 9.0/49 167 0.000 0.000 0.222 0.031 DS
82 40S ribosomal protein S12, putative Q4QG97 4.79/19 4.8/16 106 0.038 0.010 0.079 0.016 2.08
95 p21 antigen protein Q9U8C2 5.1/24 5.2/21 108 0.171 0.006 0.061 0.006 0.36
96 hypothetical protein, unknown function E9ACC9 5.04/23 5.3/18 145 0.007 0.002 0.057 0.006 8.38
101 calmodulin-like protein Q4QF91 4.8/25 4.9/21 264 0.090 0.008 0.033 0.005 0.37
110 peroxidoxin Q4QBH2 4.67/27 6.4/25 205 0.084 0.010 0.031 0.003 0.37
113 proteasome beta 6 subunit, putative Q4QJ65 4.71/29 6.5/28 174 0.163 0.037 0.068 0.007 0.42
114 triosephosphate isomerase Q4QAP8 4.77/29 8.6/27 216 0.113 0.009 0.038 0.008 0.34
119 vacuolar sorting-like protein Q4Q5H7 5.3/28 5.1/22 88 0.075 0.014 0.040 0.002 0.54
120 hs1vu complex proteolytic subunit-like Q4Q116 5.28/28 5.7/23 227 0.017 0.005 0.035 0.003 2.03
131 hypothetical protein, conserved Q4Q079 5.25/30 5.5/29 61 0.079 0.007 0.038 0.010 0.47
137 hypothetical protein, conserved (SH3 domain protein) * Q4QJ54 5.38/32 5.3/31 372 0.014 0.002 0.000 0.000 NDS
144 glycosomal malate dehydrogenase Q4QDF0 5.1/33 9.0/34 261 0.031 0.001 0.013 0.003 0.42
151 hypothetical protein, conserved Q4QED8 4.87/28 5.2/24 196 0.019 0.004 0.004 0.001 0.19
153 peroxidoxin Q4QBH2 4.58/28 4.6/25 228 0.102 0.016 0.036 0.013 0.35
154 Qc-SNARE protein, putative Q4QIG6 5.78/24 8.5/24 157 0.110 0.011 0.019 0.010 0.17
155 eukaryotic translation initiation factor 1A, putative Q4QF06 4.92/23 4.7/19 426 0.043 0.008 0.013 0.010 0.30
161 hypothetical protein, conserved (Alba superfamily) * Q4Q2T7 5.2/29 9.8/23 224 0.024 0.001 0.004 0.001 0.18
183 hypothetical protein, conserved (Alba superfamily) * Q4Q2T7 5.83/28 9.8/23 176 0.061 0.018 0.012 0.003 0.20
Spot
No. a 		Protein name Accession
No. b 		(PI/MW)
Exp. c 		(PI/MW)
Theo. d 		Protein Score. e Ave %vol Sd Ave %vol Sd Induction factor (V/C). g
203 alpha tubulin Q4QGC5 5.61/19 4.9/50 202 0.016 0.002 0.006 0.001 0.39
206 Tryparedoxin E9ADX3 5.64/16 6.6/17 40 0.018 0.001 0.037 0.007 2.11
217 proteasome alpha 1 subunit, putative E9AFW0 6.44/31 6.8/27 202 0.058 0.008 0.030 0.006 0.52
255 cytosolic malate dehydrogenase Q4Q7X6 6.4/37 5.8/34 493 0.061 0.011 0.032 0.013 0.53
273 NADP-dependent alcohol dehydrogenase, putative Q4QBD8 6.37/40 5.8/38 249 0.095 0.004 0.031 0.004 0.33
274 NADP-dependent alcohol dehydrogenase, putative Q4QBD8 6.48/40 5.8/38 366 0.287 0.031 0.146 0.019 0.51
322 co-chaperone GrpE, putative Q4Q7N4 5.89/26 7.7/24 242 0.019 0.002 0.083 0.011 4.28
324 ADP-ribosylation factor-like protein Q4Q756 5.86/23 6.0/20 169 0.053 0.015 0.017 0.001 0.32
327 hypothetical protein, unknown function Q4QEA4 6.5/21 9.3/17 46 0.000 0.000 0.027 0.007 DS
333 endoribonuclease L-PSP (pb5), putative Q4QBF5 5.96/18 5.5/17 228 0.007 0.003 0.016 0.004 2.38
350 heat shock protein-like protein, putative Q4Q584 4.9/35 5.0/36 442 0.039 0.008 0.020 0.007 0.52
353 adenylate kinase, putative Q4QC71 4.5/39 5.7/30 198 0.069 0.017 0.022 0.004 0.31
359 hypothetical protein, conserved (P-loop_NTPase super family)* Q4QFN8 5.72/36 5.6/32 85 0.074 0.013 0.030 0.012 0.40
374 succinyl-CoA synthetase alpha subunit, putative Q4Q9M4 4.77/38 9.2/31 313 0.000 0.000 0.023 0.010 DS
377 dihydroorotate dehydrogenase Q4QEW7 4.93/39 5.7/35 83 0.030 0.007 0.006 0.003 0.21
381 hypothetical protein, conserved (Enkurin superfamily) * Q4QAX0 4.65/36 9.0/31 152 0.015 0.004 0.001 0.000 0.04
389 phosphoglycerate kinase B, cytosolic Q4QD33 4.8/42 8.0/45 425 0.000 0.000 0.002 0.000 DS
414 Structure-specific endonuclease subunit SLX1 homolog Q4Q9W0 6.52/57 8.7/75 29 0.061 0.012 0.029 0.007 0.48
428 elongation factor 1-alpha Q4QEI9 6.14/47 9.0/49 202 0.058 0.005 0.009 0.002 0.16
436 succinyl-CoA ligase [GDP-forming] beta-chain, putative Q4Q1C4 5.56/33 6.5/44 345 0.000 0.000 0.047 0.012 DS
438 hypothetical protein, conserved O97202 4.1/36 9.6/46 95 0.015 0.001 0.060 0.012 3.97
449 hypothetical protein, conserved Q4QHN3 6.28/18 5.9/16 194 0.017 0.004 0.007 0.002 0.42
450 hypothetical protein, conserved Q4QHN3 6.22/19 5.9/16 152 0.015 0.005 0.103 0.025 6.75
457 peroxidoxin Q4QBH2 5.62/26 4.6/25 205 0.000 0.000 0.039 0.000 DS
466 glycosomal malate dehydrogenase Q4QDF0 5.66/33 9.0/34 217 0.024 0.004 0.000 0.000 NDS
Spot
No. a 		Protein name Accession
No. b 		(PI/MW)
Exp. c 		(PI/MW)
Theo. d 		Protein Score. e Ave %vol Sd Ave %vol Sd Induction factor (V/C). g
468 heat shock protein 90 E9ADS8 5.1/28 5.0/87 182 0.014 0.002 0.006 0.001 0.40
469 hs1vu complex proteolytic subunit-like Q4Q116 5.13/27 5.7/23 194 0.018 0.002 0.008 0.002 0.42
474 small ubiquitin protein, putative Q4QIC2 4.94/20 5.0/13 126 0.009 0.002 0.019 0.004 2.16
484 peroxidoxin Q4QBH2 4.94/27 4.6/25 226 0.044 0.007 0.022 0.008 0.50
496 hypothetical protein, conserved (SNF-7-like protein) * Q4Q130 5.02/34 4.8/25 234 0.034 0.005 0.006 0.003 0.18
a

The numbering corresponds to the 2-DE gel in Fig. 1./

b

Accession number in Swiss-Prot. /

c

Experimental pI and molecular weight.

d

Theoretical pI and molecular weight./

e

Mascot score greater than 26 were significant at p=0.05./

F

Expression pattern of spots that showed a statistically change in cutaneous and visceral leishmaniasis./

g

The induction factor calculated by dividing the percent volume of spots in gels corresponding to visceral leishmaniasis to the percent volume of spots in cutaneous leishmaniasis samples./ Average percent volume (Ave %vol) and standard deviation (sd) of spots from two samples (cutaneous and visceral) and Three replication have been presented. Spots were concluded to be significantly up- or down-regulated when p was <0.05./

*

Hypothetical proteins with special domains /The data-base search was run against the NCBI non-redundant protein data-base and Uniprot's Swiss-Prot.