Table 3.
Functional and Biological process categories of the identified proteins
| Spot No a | Protein name | Accession No b | Induction factor (V/C) c | ||
|---|---|---|---|---|---|
| Carbohydrate metabolic process/Energy metabolism | |||||
| 114 | triosephosphate isomerase | Q4QAP8 | 0.34 | ||
| 131 | hypothetical protein, conserved (ATP synthase B super family) * | Q4Q079 | 0.47 | ||
| 144 | glycosomal malate dehydrogenase | Q4QDF0 | 0.42 | ||
| 255 | cytosolic malate dehydrogenase | Q4Q7X6 | 0.53 | ||
| 273 | NADP-dependent alcohol dehydrogenase, putative | Q4QBD8 | 0.33 | ||
| 274 | NADP-dependent alcohol dehydrogenase, putative | Q4QBD8 | 0.51 | ||
| Protein synthesis/assembly | |||||
| 58 | hypothetical protein, conserved (Alba superfamily) * | Q4QGA9 | 0.37 | ||
| 155 | eukaryotic translation initiation factor 1A, putative | Q4QF06 | 0.30 | ||
| 161 | hypothetical protein, conserved (Alba superfamily) * | Q4Q2T7 | 0.18 | ||
| 183 | hypothetical protein, conserved (Alba superfamily) * | Q4Q2T7 | 0.20 | ||
| 428 | elongation factor 1-alpha | Q4QEI9 | 0.16 | ||
| Cell signaling and vesicular trafficking | |||||
| 101 | calmodulin-like protein | Q4QF91 | 0.37 | ||
| 119 | vacuolar sorting-like protein | Q4Q5H7 | 0.54 | ||
| 154 | Qc-SNARE protein, putative | Q4QIG6 | 0.17 | ||
| 324 | ADP-ribosylation factor-like protein | Q4Q756 | 0.32 | ||
| 381 | hypothetical protein, conserved (Enkurin superfamily) * | Q4QAX0 | 0.04 | ||
| 496 | hypothetical protein, conserved (SNF-7-like protein) * | Q4Q130 | 0.18 | ||
| Intracellular survival/Proteolysis | |||||
| 120 | hs1vu complex proteolytic subunit-like | Q4Q116 | 2.03 | ||
| 333 | endoribonuclease L-PSP (pb5), putative | Q4QBF5 | 2.38 | ||
| 474 | small ubiquitin protein, putative | Q4QIC2 | 2.16 | ||
| Antioxidant defense/Oxidative stress defense | |||||
| 206 | Tryparedoxin | E9ADX3 | 2.11 | ||
| 457 | peroxidoxin | Q4QBH2 | DS | ||
| Stress related proteins/protein folding | |||||
| 322 | co-chaperone GrpE, putative | Q4Q7N4 | 4.28 | ||
| Cell motility and cytoskeleton | |||||
| 44 | ADF/Cofilin | E9ADQ2 | 0.21 | ||
| 203 | alpha tubulin | Q4QGC5 | 0.39 | ||
| Nucleoside, nucleotide and nucleic acid metabolism | |||||
| 353 | adenylate kinase, putative | Q4QC71 | 0.31 | ||
| 377 | dihydroorotate dehydrogenase | Q4QEW7 | 0.21 | ||
| Cell duplication/Cell cycle | |||||
| 70 | hypothetical protein, conserved (Sfi1 spindle body protein) * | Q4Q6E8 | DS | ||
| Table 3. (Continued). | |||||
| Spot No a | Protein name | Accession No b | Induction factor (V/C) c | ||
| Diverse cellular functions | |||||
| 359 | hypothetical protein, conserved (P-loop_NTPase super family) * | Q4QFN8 | 0.40 | ||
| DNA damage/DNA recomibination/DNA repair | |||||
| 414 | Structure-specific endonuclease subunit SLX1 homolog | Q4Q9W0 | 0.48 | ||
| Hypotical protein/unknown function | |||||
| 28 | hypothetical protein | Q4QFW1 | 0.51 | ||
| 36 | hypothetical protein | Q4QFW1 | NDS | ||
| 96 | hypothetical protein, unknown function | E9ACC9 | 8.38 | ||
| 151 | hypothetical protein, conserved | Q4QED8 | 0.19 | ||
| 327 | hypothetical protein, unknown function | Q4QEA4 | DS | ||
| 438 | hypothetical protein, conserved | O97202 | 3.97 | ||
| 449 | hypothetical protein, conserved | Q4QHN3 | 0.42 | ||
| 450 | hypothetical protein, conserved | Q4QHN3 | 6.75 | ||
a
The numbering corresponds to the 2-DE gel in Fig. 1./
b
Accession number in Swiss-Prot./
g
The induction factor calculated by dividing the percent volume of spots in gels corresponding to visceral leishmaniasis to the percent volume of spots in cutaneous leishmaniasis samples./ DS: detected spots only in visceral leishmaniasis, NDS: not detected spots in visceraleishmaniasis./ Significantly up-regulated proteins in visceral leishmaniasis compared to cutaneous leishmaniasis./ Significantly down-regulated proteins in visceral leishmaniasis compared to cutaneous leishmaniasis.
*
Hypothetical proteins with special domains