Table 1.

Primers used for amplification of pfcrt gene fragment of 145 base pairs

PCR Round	Primer	Primer Sequence (5′–3′)	Tm
1st Round	Crtd-A (forward primer)	5′-GGTGGAGGTTCTTGTCTTGGTA-3′	57.5 °C
	Crtd-B (reverse primer)	5′-GACCTATGAAGGCCAAAATGACTG-3′	56.7 °C
2nd Round	Crtd-C (forward primer)	5′-TGTGCTCATGTGTTTAAACTT-3′	50.06 °C
	Crtd –D (reverse primer)	5′-CAAAACTATAGTTACCAATTTT-3′	46.10 °C