Table 1.

Targeted pathway, chemical, and experimental result. All chemicals were tested at 10 uM unless otherwise noted:

Pathway/Process	Chemical	Result Prob
Iron chelation	ciclopirox	Toxic
Myosin II ATPase	(−)-blebbistatin	Toxic
PI3K	wortmannin	Toxic
Protein secretion / trafficking	brefeldin A	Toxic
Ros Inhibitor	apocynin	Toxic
Wnt	tideglusib	Toxic
Wnt	TWS119	Toxic
WNT/GSK3	BIO3	Toxic
EGFR	gefitinib	NS
FGFR	danusertib	NS
FGFR	AZD4547	NS
MMP	doxycycline	NS
Multiple biological effects	dexamethasone2	NS
Notch	semagacestat	NS
Notch	MG-132	NS
Protein translation	azithromycin	NS
Stat1	fludarabine	NS
TNFa	lenalidomide	NS
V-ATPase H+ Inhibitor	concanamycin A6	NS
Wnt	ICG-001	NS
Wnt	KY02111	NS
Wnt	agonist I	NS
Wnt	nicotine	NS
Apoptosis	NS3694	< 0.05
Hedgehog	vismodegib	< 0.05
Multiple biological effects	luteolin	< 0.05
Wnt	agonist II SKL	< 0.05
Wnt	XAV-9391	< 0.05
Wnt	C59	< 0.01
Wnt	IWR-1-endo	< 0.01
Fgfr	BGJ398	< 0.002
Multiple biological effects	dexamethasone Na phosphate	< 0.002
Tgfβ/activin	SB-505124	< 0.002
Wnt	C594,5	< 0.002

¹40.0 uM;

²25.0 uM;

³15.0 uM;

⁴5.0 uM;

⁵2.5 uM;

⁶1.0 uM.

Prob is the probability that the proportional change in post-amputation body length is significantly different between control and chemically treated embryos. NS = not significant. The probability value 0.002 is the Bonferroni correction for multiple testing.