(A) DAVID pathway analysis of down-regulated genes in BaLKO mouse livers identified by microarray.
(B) Heat map representation of genes involved in bile acid metabolism.
(C-E) qPCR analysis of the mRNA expression of transcriptional factors (C), CAR target genes (D), and FXR, LXR and PXR target genes (E) involved in bile acid metabolism in livers from flox/flox and BaLKO mice fed with WD for 12 weeks (n = 4-5).
(F) Plasma concentrations of Pcsk9 (n = 4-5).
(G) Immunoblots of total liver lysates. Data in C-F are mean ± s.e.m.; *P < 0.05 by two-tailed Student's t-test.
See also Figure S2.