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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Biomaterials. 2015 Oct 14;76:344–358. doi: 10.1016/j.biomaterials.2015.10.020

Figure 2. Immobilization of heparin and VEGF on SIS.

Figure 2

(a) Binding of heparin on SIS. iHep: covalently bound heparin using EDC/NHS chemistry; aHep: absorbed or physisorbed heparin. Representative bright field images of SIS are shown for each condition with bound toluene blue dye. Size of SIS is 2mm × 2mm. (b) VEGF immobilization on SIS as measured by direct ELISA. (a, b) The symbols (# and @) denote statistical significance as compared to the other two conditions. (c) VEGF release from SIS over time. Elution of VEGF from unmodified SIS or SIS with iHep in PBS with the indicated heparin concentrations or EGM medium containing serum. At the indicated times, the VEGF that was released in solution was measured by ELISA. The symbol (*) denotes statistical significance of all iHep from all SIS only samples.