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. Author manuscript; available in PMC: 2015 Nov 28.
Published in final edited form as: Science. 2015 Oct 15;350(6264):1096–1101. doi: 10.1126/science.aac7041

Fig. 3. Functional characterization of novel cell-essential genes.

Fig. 3

(A) Of the 1,878 cell-essential genes identified in the KBM7 cell line, 330 genes had no known function. (B) Genes co-expressed with C16orf80, C3orf17, and C9orf114 across CCLE cell lines were associated with RNA processing. Parentheses denote the number of genes in the set. (C) Proliferation of KBM7 cells transduced with sgRNAs targeting C16orf80, C3orf17, and C9orf114, or a non-targeting control. Error bars denote SD (n=4). (D) C16orf80 localized to the nucleus and C3orf17 and C9orf114 to the nucleolus. Fibrillarin-RFP was used as a nucleolar marker. (E) Multiple subunits of the spliceosome, RNAse P/MRP and H/ACA ribonucleoprotein complexes interact with C16orf80, C3orf17, and C9orf114, respectively.