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. Author manuscript; available in PMC: 2016 Nov 12.
Published in final edited form as: Wound Repair Regen. 2015 Oct 19;23(6):785–796. doi: 10.1111/wrr.12346

Figure 6. CXCR3 ligands promote transmigration.

Figure 6

Pericytes (muscle, MG-136) were analyzed for their ability to migrate through a Matrigel coated transwell (8.0 μm pore). Pericytes were suspended in 0.1% dialyzed FBS-media and plated in the upper chamber. The lower chamber contained 0.1% dialyzed FBS-media with CXCR3 ligands only or SB203580 (10 μM) or PD98059 (10 μM). The cells were incubated for 48 hrs A.) CXCR3 ligands enhance pericyte migration compared to diluent (NT). B.) Treatment of the cells with SB203580 (SB), P38MAPK inhibitor, significantly inhibited transmigration compared to PD98059 or diluent. These data suggest P38MAPK is involved in CXCR3 mediated transmigration. Shown are mean ± s.e.m of three experiments each in duplicate (n=3). * P < 0.05 comparing NT to CXCR3 ligand treated and untreated with inhibitor treatment.