Fig. 4.
Over-expression of OSC can protect HL-02 cells from the injury of IH. After transfection, HL-02 cells were exposed to IH (cycle of 2 % O2 5 min and 21 % O2 10 min) or normoxia (21 % O2) for 4 days. a Protein levels of OSC in HL-02 cells transfected with pEGFP-N1 or OSC-pEGFP were determined by Western blot after IH treatment. b Effect of the IH on the cell viability in HL-02 cells transfected with pEGFP-N1 or OSC-PGFP. c Representative flow cytometry profiles of cell apoptosis probed by annexin V binding (horizontal) and PI exclusion (vertical) in HL-02 cells transfected with pEGFP-N1 or OSC-EGFP after IH treatment. d Percentages of apoptotic HL-02 cells transfected with pEGFP-N1 or OSC-EGFP after IH treatment. *p < 0.05 vs the cells transfected with pEGFP-N1 control plasmid