Figure 2.

Dependence of IAPP self-association on sample conditions. (a) IAPP aggregation is strongly dependent on the formation of micelles occurring at specific critical micellar concentrations. (b) Mapping of the self-association of hIAPP as a function of pH and temperature. (Top) The micelle is not formed when H18 is ionized and no self-association is observed below pH 6. (Middle) At neutral pH, but below a critical temperature for micelle formation (4°C), small oligomers form which predominantly interact with the N-terminal helix (residues 7–16). (Bottom) Above the temperature for micelle formation (10°C), self-association is found throughout the peptide but particularly in the central region (residues 12–21). Adapted from [21].