Table 1. Mechanical Properties of Cantilevers.
type of cantilever | L (μm)a | k (pN/nm)b | fc (kHz)c | Qc | τcalc (μs)d | τ (μs)e |
---|---|---|---|---|---|---|
Long BioLever | 100 | 4.3 | 1.2 ± 0.1 | 0.32 ± 0.02 | 86 | 450f |
Modified BioLever Mini | 38 | 4.7 | 4.1 ± 0.1 | 0.325 ± 0.004 | 25 | 53f |
BioLever Fast | 9 | 130 | 444 ± 2 | 0.847 ± 0.003 | 0.6 | 0.4 |
Stiff modified BioLever Fast | 9 | 40 | 240 ± 6 | 0.52 ± 0.01 | 0.7 | 1.2 |
Soft modified BioLever Fast | 9 | 20 | 175 ± 3 | 0.43 ± 0.01 | 0.8 | 1.8 |
Typical length provided by manufacturer.
Spring constant measured for each cantilever as described in Supporting Information.
Extracted from a fit of the simple harmonic oscillator equation to the power spectral densities measured 50 nm above the surface in liquid.
τcalc ≅ (Q/πfc).34 However, this metric should be used with caution since it differed substantially from the measured relaxation time τ.
Measured τ determined from exponential fit to the tip–sample detachment during SMFS experiments.
See ref (31).