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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Oct 23;1858(1):85–96. doi: 10.1016/j.bbamem.2015.10.009

Fig 3. n-3 PUFA decrease the interaction of fluorescent non-raft and PI(4,5)P2 markers detected using FRET in Fat-1 CD4+ T cells.

Fig 3

(A and B) Schematic diagrams of the interaction between neighboring fluorescent probes targeted to the non-raft membrane fraction of the plasma membrane, and PH(PLC-δ), a PI(4,5)P2 reporter, or the control PHR40L(PLC-δ). CD4+ T cells were incubated with lentivirus containing (C) Src(N15)/PH(PLC-δ) (n = 7 per genotype), or (D) Src(N15)/PHR40L(PLC-δ) (n = 4 per genotype) before FRET by acceptor photobleaching. FRET efficiency (E%) was determined as described in Fig 1 (*P < 0.05 between genotypes). (E) CD4+ T cells were isolated and transduced with Src(N15)-ECFP and PH(PLC-δ)-YPet for 48 hrs prior to incubation with 1.25 μM PI(4,5)P2 or PBS (0 μM) for one hr. Cells were analyzed as described in Fig 1. A two-tailed t-test was used to compare FRET efficiencies within specific concentrations (*P < 0.05 at specific concentrations of PI(4,5)P2).