Figure 5.

Loss of DUOX activity disturbs mucosal homeostasis in the terminal ileum. (A) Experimental setup for gene expression profiling. (B) Expression heat maps depicting selected genes upregulated in the ileum of Duoxa^−/− animals. (C) Ileal gene expression analyzed by RT-qPCR. Mean expression in Duoxa^−/− animals is plotted relative to the mean in cohoused wt littermates (set to 1) in cage-wise comparisons (n=7). **, P<.01. (D, E) Genes controlled by SFB-monocolonization or by cohousing of B6-Jax (SFB^neg) with B6-Tac (SFB^pos) mice ¹⁵ were tested for correlation with genes affected by loss of DUOX activity using GSEA (see Supplementary Methods). Within each plot, genes are sorted for their relative ileal expression in Duoxa^−/− mice (left side: up in Duoxa^−/−; right side: down in Duoxa^−/−). Genes upregulated (downregulated) by introduction of SFB are significantly correlated with those upregulated (downregulated) in mice with loss of DUOX activity. Core enriched genes are listed in Supplementary Tables S16-S19. FDR, false discovery rate q-value; NES, normalized enrichment score.