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. 2015 Nov 16;2016:5710403. doi: 10.1155/2016/5710403

Table 2.

Characteristics of allelic variants and some conditions for their detection.

Gene polymorphisms Primer sequences Restriction enzyme PCR products (bp)
Nucleotide excision repair

ERCC2/XPD Asp312Asn rs1799793 (F) 5′-CTG TTG GTG GGT GCC CGT ATC TGT TGG TCT-3′  
(R) 5′-TAA TAT CGG GGC TCA CCC TGC AGC ACT TCC T-3′
StyI Asp/Asp: 507 + 244;
Asp/Asn:  507 + 474 + 244 + 33;
Asn/Asn: 474 + 244 + 33

ERCC2/XPD Lys751Gln rs13181 (F) 5′-GCC CGC TCT GGA TTA TAC G-3′  
(R) 5′-CTA TCA TCT CCT GGC CCC C-3′
Pst I Lys/Lys: 290 + 146;
Lys/Gln: 290 + 127 + 146;
Gln/Gln: 227 + 146

ERCC6/CSB Met1097Val rs2228526 (F) 5′-CCT GCT T CT AAC ATA TCT GT-3′  
(R) 5′-AAT CAC TGA CAA CTC TTC TG-3′
Nla III Met/Met: 123 + 78;
Met/Val: 201 + 123 + 78;
Val/Val: 201

ERCC6/CSB Gly399Asp rs2228528 (F) 5′-TGA AGA GTC TGA GTA TTT CC-3′  
(R) 5′-ATC TTC ATC TCC ATC ATC TC-3′
RsaI Gly/Gly: 180 + 91;
Gly/Asp: 271 + 180 + 91;
Asp/Asp: 271

Base excision repair

XRCC1
Arg399Gln  
rs25487
(F) 5′-GGA CTG TCA CCG CAT GCG TCG G-3′  
(R) 5′-GGC TGG GAC CAC CTG TGT T-3′
MspI Arg/Arg: 115 + 34;
Arg/Gln: 149 + 115 + 34;
Gln/Gln: 149

OGG1
Ser326Cys
rs1052133
(F) 5′-CTG TTC AGT GCC GAC CTG CGC CGA-3′  
(R) 5′-ATC TTG TTG TGC AAA CTG AC-3′
MboI Ser/Ser: 224 + 23;
Ser/Cys: 247 + 224 + 23;
Cys/Cys: 247