Table 1.
Gene | PolB | LIG3 | EXO1 |
---|---|---|---|
Polymorphism | 242Pro/Arg | 780Arg/His | 589Lys/Glu |
| |||
refSNP | 3136797 | 3136025 | 1047840 |
| |||
Thermal conditions | (1) 95°C—10 min | ||
(2) 92°C—15 sec | |||
(3) 60°C—1 min | |||
(4) Step 2 and 3—45x | |||
| |||
Dyes | ROX, HEX, and FAM | ||
| |||
Ref. dye | ROX |