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. 2015 Nov 16;2016:3125989. doi: 10.1155/2016/3125989

Table 1.

The refSNP and thermal conditions used in the PCR reaction.

Gene PolB LIG3 EXO1
Polymorphism 242Pro/Arg 780Arg/His 589Lys/Glu

refSNP 3136797 3136025 1047840

Thermal conditions  (1) 95°C—10 min
(2) 92°C—15 sec
(3) 60°C—1 min
   (4) Step  2 and 3—45x

Dyes ROX, HEX, and FAM

Ref. dye ROX