Figure 3.

Effect of OP9 or OP9DL1 on differentiation of bone marrow CD34⁺ cells. OP9 or OP9DL1 cells (5 × 10⁴/well) were plated in 12-well plates with α-MEM medium plus 20% FBS 12 h prior to the addition of BM CD34⁺ (5 × 10⁵/well). The coculture was started with α-MEM medium plus 20% FBS containing a final concentration of 5 ng/mL each of IL-7 and Flt-3 ligand (Flt-3L). 12 days later, cells were collected for FACS analysis ((a) and (b)), and EBF1 or GATA3 gene expression was determined by real-time PCR after 5-day coculture, respectively ((c) and (d)). (Error bars present the SD of the mean values, ^∗∗ P < 0.01.)