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AICAR induces AMPK activation in HL-1 cardiac cells. HL-1 cells were stimulated with AICAR for 1 h and the AMPK activation was confirmed by the protein levels of phospho-Thr172-AMPK (a) and phospho-Ser79-ACC (b). Quantifications show the ratio between phosphorylated and total forms of each protein. Data are expressed as mean ± SD of 3 different experiments. (∗ P < 0.05, ∗∗ P < 0.01 versus control nonstimulated cells).
