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. 2015 Nov 16;2015:785783. doi: 10.1155/2015/785783

Figure 4.

Figure 4

AICAR prevents the HP/HI-induced intramyocellular lipid accumulation in HL-1. Lipid content was analyzed by Oil-Red-O staining in HL-1 challenged with HP/HI for 16 h in the presence or absence of AICAR (24 h). (a) Representative microphotography showing lipid droplets in cells counterstained with Haematoxylin (bar 20 μm). Squares indicate the areas shown at high magnification. (b) Quantification of stained areas relative to cell surface. Data are expressed as mean ± SD of 5 different pictures from 3 independent experiments (∗∗ P < 0.01 versus control nonstimulated cells; # P < 0.05 versus HP/HI-stimulated cells).