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. 2015 Nov 16;2016:4512309. doi: 10.1155/2016/4512309

Figure 2.

Figure 2

Effects of acetaldehyde treatment on the activation of Akt and CREB in SH-SY5Y cells. SH-SY5Y cells were treated with different concentrations of acetaldehyde for 24 h. Total cell lysates were collected and the amount of Akt, phospho-Akt (Ser473) (a), CREB, and phospho-CREB (Ser 133) (b) was determined by Western blot analysis. The intensities of the bands were quantified by densitometric analyses and normalized by the amount of Akt or CREB. Values are means ± SD from three independent experiments. ∗, significantly different from control (P < 0.05); ∗∗, significantly different from control (P < 0.01).