Figure 6. BA physiology is diversely altered in Insr^P1195L/+/HFD liver.

(a–d) mRNA expressions of genes involved in BA synthesis (n = 8–12 per each group). (e,f) mRNA expressions of genes involved in BA transport (n = 8–12 per each group). (g) Relative BA composition in liver in refed condition (n = 3). (h) The ratio of muricholates to cholates, calculated with the molar percentage of tauro α-, tauro β-, and tauro ω-mucricholates and taurocholate. (i) mRNA expression of Cyp7a1 after oral glucose loading in Kir6.2^−/− mice (n = 7–12 per each group). (j) mRNA expression of Cyp7a1 in fat transplanted Insr^P1195L/+/HFD mice (n = 5–7 per each group). (k) mRNA expression of Cyp7a1 in the WT liver after intraperitoneal glycerol administration (n = 7–10 per each group). Data are mean ± SEM. Two-way ANOVA plus Bonferroni post-hoc analysis (a–f). Significance between treatment by two-tailed Student’s t-test (i). One-way ANOVA plus Bonferroni post-hoc analysis (g,h,j,k). *P < 0.05, **P < 0.01, ***P < 0.001, NS; not significant.