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. 2015 Nov 30;5:17228. doi: 10.1038/srep17228

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(A) NIH3T3 cells were infected with M1-cHA MHV-68 at MOI 10 and harvested at indicated time points for western blot analysis using antibodies specific for tublin (top), HA (middle), ORF26 and M9 (bottom). (B) NIH3T3 cells were mock-infected or infected with wild-type (WT) or M1-cHA MHV-68 at MOI 10 and fixed for IFA analysis as described in Materials and Methods using antibodies against HA (red), concanavaline A (conc A) (green) and Hoechst (Blue).