Figure 4.

Recalcification time and inhibition assay. RBCs were treated with different concentrations of IS or IAA for 24 h, cells and RMPs were collected, respectively. KCl or ethanol was utilized as their respective controls. Coagulation times of 100 μL RBCs (1 × 10⁸) and RMPs (prepared from 10 mL of the RBCs supernatants) in each group of IS (A) and IAA (B) are shown. PCA of RBCs and RMPs that incubated in IS (C) and IAA (D) were detected in the absence or presence of 128 nM lactadherin. Data are displayed as mean ± SD for triplicate samples of independent experiments, * indicate p < 0.001.