Table 2.
Cytotoxicity and toxin titers. All strains have been tested by the Vero, as well as CaCo-2 cytotoxicity assay, an in-house sandwich EIA, and two in-house indirect EIAs. Titers indicated are means and SD of triplicates. The strains reacted positively in the Duopath® test; Duopath® tests were performed twice.
| Strain | Cytotoxicity titer (Vero cells) | Cytotoxicity titer (CaCo-2 cells) | Sandwich EIA titer | Indirect EIA 1E11 titer | Indirect EIA 2B11 titer | Duopath® |
|---|---|---|---|---|---|---|
| MHI 241 | 1381 (±8.0) | 358 (±46.2) | 27072 (±2841.9) | 2669 (±128.7) | 118 (±0.7) | + |
| MHI 3178 | 16 (±4.2) | 4 (±0.3) | 192 (±7.8) | 113 (±9.2) | 27 (±2.8) | + |
| MHI 1440 | 218 (±20.5) | 202 (±39.2) | 5.4 (±0.2) | 583 (±33.9) | 2.6 (±1.3) | + |
| MHI 1541 | 533 (±46.0) | 198 (±22.4) | 19 (±1.4) | 775 (±76.4) | 6.7 (±1.1) | + |
| MHI 1668 | 628 (±36.8) | 157 (±26.6) | 47.5 (±0.7) | 2330 (±753.1) | 47.5 (±0.7) | + |
| MHI 2970 | 755 (±4.2) | 345 (±80.2) | 41.5 (±2.1) | 1204 (±125.2) | 11 (±1.3) | + |
| MHI 3173 | 1210 (±253.1) | 271 (±46.3) | 38.5 (±2.7) | 1682 (±14.1) | 5.7 (±2.0) | + |
| MHI 3225 | 1274 (±247.5) | 200 (±10.2) | 36.5 (±0.7) | 1692 (±14.1) | 6.9 (±1.3) | + |
| MHI 1430 | 679 (±89.0) | 280 (±15.4) | 467 (±26.9) | 2383 (±501.3) | 51 (±9.9) | + |
| MHI 1444 | 740 (±162.3) | 508 (±60.9) | 445 (±85.6) | 2591 (±413.0) | 47 (±2.8) | + |
| MHI 1758 | 1053 (±215.0) | 699 (±109.0) | 961 (±56.6) | 2755 (±166.2) | 52 (±4.2) | * |
+ positive reaction; * not analyzed.