Table 2.
Proton chemical shifts (ppm) of the Vibrio alginolyticus exopolysaccharide (VA-EPS) and the purified oligosaccharides (DP4, DP8). A–D refer to the residues composing the repetition moieties of the polysaccharide. When the residues are located at the reducing end or at the non-reducing end, they are labelled r and nr, respectively.
| VA-EPS: [ABCD]n | DP4: AnrBCr′Dr | DP8: AnrBCDABCr′Dr | |
|---|---|---|---|
| A1: 6Ala-GalA/A2:6Ser-GalA | |||
| H1 | 5.18/5.19 | 5.20/5.20 | |
| H2 | 3.84/3.84 | 3.80/3.80 | |
| H3 | 4.00/4.00 | 4.04/4.04 | |
| H4 | 4.49/4.51 | 4.54/4.54 | |
| H5 | 4.91/4.96 | 4.99/5.04 | |
| A1nr: 6Ala-GalA/A2nr:6Ser-GalA | |||
| H1 | 5.22/5.23 | 5.20/5.22 | |
| H2 | 3.78/3.78 | 3.80/3.80 | |
| H3 | 3.97/3.99 | 3.99/3.99 | |
| H4 | 4.33/4.36 | 4.34/4.34 | |
| H5 | 4.99/5.04 | 4.99/5.04 | |
| B: GalA | |||
| H1 | 5.22 | 5.22 | 5.23 |
| H2 | 3.93 | 3.93 | 3.95 |
| H3 | 4.10 | 4.14 | 4.15 |
| H4 | 4.51 | 4.52 | 4.53 |
| H5 | 4.58 | 4.64 | 4.65 |
| C: 6Ala-GalA | |||
| H1 | 5.43 | 5.50 | |
| H2 | 3.97 | 3.98 | |
| H3 | 4.03 | 4.02 | |
| H4 | 4.48 | 4.50 | |
| H5 | 4.31 | 4.32 | |
| Cr′: 6Ala-GalA | |||
| H1-Dr α/β | 5.48/5.51 | 5.48/5.51 | |
| H2-Dr α/β | 3.98/3.97 | 3.98/3.97 | |
| H3-Dr α/β | 4.02/4.00 | 4.03/4.00 | |
| H4 | 4.50 | 4.50 | |
| H5 | 4.31 | 4.32 | |
| D: GlcNAc | |||
| H1 | 4.77 | 4.75 | |
| H2 | 3.85 | 3.87 | |
| H3 | 3.81 | 3.87 | |
| H4 | 3.70 | 3.75 | |
| H5 | 3.51 | 3.53 | |
| H6′ | 3.93 | 3.95 | |
| H6′ | 3.77 | 3.82 | |
| NHAc:CH3 | 1.95 | 1.95 | |
| Dr: GlcNAc | |||
| H1 α/β | 5.18/4.73 | 5.18/4.73 | |
| H2 α/β | 4.03/3.80 | 4.03/3.81 | |
| H3 α/β | 3.99/3.81 | 4.00/3.81 | |
| H4 α/β | 3.75/3.73 | 3.75/3.73 | |
| H5 α/β | 3.92/3.52 | 3.93/3.53 | |
| H6 α/β | 3.84/3.91 | 3.95/3.82 | |
| H6′ | 3.78 | 3.82 | |
| NHAc: CH3 | 1.95 | 1.95 | |