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. 2015 Nov 9;13(11):6774–6791. doi: 10.3390/md13116774

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Effect of fascaplysin on leukocytic CD11b expression. (A–C) Whole blood was incubated with 10 μM fascaplysin (black bars, n = 6) or vehicle (DMSO, white bars, n = 6) for 0.5 h followed by stimulation with PAR-1-AP, ADP or PMA. Data are given in % of vehicle. Unstimulated whole blood served as negative control (grey bars, n = 6). Expression of CD11b on all leukocytes (A), monocytes (B) and granulocytes (C) were assessed by flow cytometry using double fluorescence staining (CD45/CD11b). Mean ± SEM. * p < 0.05 vs. vehicle.