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. 2015 Nov 9;13(11):6774–6791. doi: 10.3390/md13116774

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Effect of fascaplysin and heparin on thrombus formation and tail-vein bleeding time. (A) Intravital fluorescent microscopic images of a postcapillary venule in the dorsal skinfold chamber of a vehicle-treated BALB/c mouse before (baseline) and after photochemically induced thrombus formation (asterisk). Blue-light epi-illumination with contrast enhancement by 5% fluorescein isothiocyanate (FITC)-labeled dextran 150,000. Scale bar: 50 μm. (B) Complete occlusion time of postcapillary and collecting venules upon photochemically induced thrombus formation in dorsal skinfold chambers of heparin-treated (upper black bar, n = 3), fascaplysin-treated (lower black bar, n = 6), and vehicle-treated BALB/c mice (upper white bar, saline, n = 3 and lower white bar, DMSO, n = 6), as assessed by intravital fluorescence microscopy. Mean ± SEM. * p < 0.05 vs. vehicle. (C) Tail-vein bleeding time of heparin-treated (upper black bar, n = 3), fascaplysin-treated (lower black bar, n = 6), and vehicle-treated BALB/c mice (upper white bar, saline, n = 3 and lower white bar, DMSO, n = 6). Mean ± SEM. * p < 0.05 vs. vehicle.

Effect of fascaplysin and heparin on thrombus formation and tail-vein bleeding time. (A) Intravital fluorescent microscopic images of a postcapillary venule in the dorsal skinfold chamber of a vehicle-treated BALB/c mouse before (baseline) and after photochemically induced thrombus formation (asterisk). Blue-light epi-illumination with contrast enhancement by 5% fluorescein isothiocyanate (FITC)-labeled dextran 150,000. Scale bar: 50 μm. (B) Complete occlusion time of postcapillary and collecting venules upon photochemically induced thrombus formation in dorsal skinfold chambers of heparin-treated (upper black bar, n = 3), fascaplysin-treated (lower black bar, n = 6), and vehicle-treated BALB/c mice (upper white bar, saline, n = 3 and lower white bar, DMSO, n = 6), as assessed by intravital fluorescence microscopy. Mean ± SEM. * p < 0.05 vs. vehicle. (C) Tail-vein bleeding time of heparin-treated (upper black bar, n = 3), fascaplysin-treated (lower black bar, n = 6), and vehicle-treated BALB/c mice (upper white bar, saline, n = 3 and lower white bar, DMSO, n = 6). Mean ± SEM. * p < 0.05 vs. vehicle.