Table 2.

Plackett-Burman design for the screening of critical media components involved in fucoidanase activity.

Runs	Real Levels (Coded Levels)/Concentrations (g/L)						Fucoidanase Activity (U/mL)
	X1	X2	X3	X4	X5	X6	Observed a	Predicted
1	5 (+1)	0.1 (−1)	0.5 (+1)	0.01 (−1)	0.01 (−1)	0.1 (−1)	2.83	2.78
2	5 (+1)	1.0 (+1)	0.1 (−1)	0.05 (+1)	0.01 (−1)	0.1 (−1)	7.71	7.74
3	1 (−1)	1.0 (+1)	0.5 (+1)	0.01 (−1)	0.05 (+1)	0.1 (−1)	2.42	2.25
4	5 (+1)	0.1 (−1)	0.5 (+1)	0.05 (+1)	0.01 (−1)	1.0 (+1)	12.56	12.21
5	5 (+1)	1.0 (+1)	0.1 (−1)	0.05 (+1)	0.05 (+1)	0.1 (−1)	10.62	10.60
6	5 (+1)	1.0 (+1)	0.5 (+1)	0.01 (−1)	0.05 (+1)	1.0 (+1)	8.91	9.36
7	1 (−1)	1.0 (+1)	0.5 (+1)	0.05 (+1)	0.01 (−1)	1.0 (+1)	8.25	8.82
8	1 (−1)	0.1 (−1)	0.5 (+1)	0.05 (+1)	0.05 (+1)	0.1 (−1)	8.39	7.95
9	1 (−1)	0.1 (−1)	0.1 (−1)	0.05 (+1)	0.05 (+1)	1.0 (+1)	14.86	15.09
10	5 (+1)	0.1 (−1)	0.1 (−1)	0.01 (−1)	0.05 (+1)	1.0 (+1)	12.81	12.77
11	1 (−1)	1.0 (+1)	0.1 (−1)	0.01 (−1)	0.01 (−1)	1.0 (+1)	7.37	6.52
12	1 (−1)	0.1 (−1)	0.1 (−1)	0.01 (−1)	0.01 (−1)	0.1 (−1)	2.13	2.80

^a The observed values of fucoidanase production were the mean values of duplicates.