Table 3.

Experimental design and results of the Box-Behnken optimization experiment.

Trials	X1	X2	X6	Fucoidanase Activity (U/mL)
				Observed a	Predicted
1	1	0.10	0.55	3.71	3.73
2	5	0.10	0.55	5.83	5.84
3	1	1.00	0.55	7.92	7.91
4	5	1.00	0.55	10.88	10.86
5	1	0.55	0.10	9.89	9.81
6	5	0.55	0.10	13.56	13.48
7	1	0.55	1.00	14.38	14.46
8	5	0.55	1.00	15.76	15.85
9	3	0.10	0.10	8.51	8.57
10	3	1.00	0.10	11.36	11.46
11	3	0.10	1.00	10.47	10.37
12	3	1.00	1.00	16.74	16.68
13	3	0.55	0.55	14.52	14.75
14	3	0.55	0.55	14.83	14.75
15	3	0.55	0.55	14.79	14.75
16	3	0.55	0.55	14.81	14.75
17	3	0.55	0.55	14.80	14.75

^a The observed values of fucoidanase production were the mean values of duplicates.